Protein_Domain

Part:BBa_K1422500:Design

Designed by: Casey Martin   Group: iGEM14_Purdue   (2014-10-13)


Zea mays S-adenosylmethione synthase (corn SAM synthase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 374
    Illegal PstI site found at 860
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 820
    Illegal PstI site found at 374
    Illegal PstI site found at 860
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 310
    Illegal XhoI site found at 1194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 374
    Illegal PstI site found at 860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 374
    Illegal PstI site found at 860
    Illegal NgoMIV site found at 198
    Illegal NgoMIV site found at 993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1197


Design Notes

This sequence is codon optimized for Bacillus subtilis (the difference being that the 368th codon was changed from AGG to AGA).

Source

It comes from corn and was found on the NCBI website. NCBI. (2014). S-adenosylmethionine synthetase 1 [Zea mays]. http://www.ncbi.nlm.nih.gov/protein/NP_001148708.1

References